VCOM Louisiana Research Day Program Book 2024

lipophilicity, and overall drug-likeness. Also, molecular docking was done to attain the binding energy of synthesized compounds to possible biomarkers/targets for further testing. The 23 compound library series were screened on A549 cells using 10% MTT assay at concentration of 1X 104. From maximum concentration of 100uM to lowest concentration of 0.00039uMWe were able to identify 5 active and potent compounds which were screened on non-cancerous HLF cells at timed intervals of 24hrs, 48hrs, 72hrs to assess its toxicity and selectivity concentrations towards non cancerous cells. Conclusions: Non-small cell lung cancer remains the principal cause of cancer death worldwide with considerable rise in annual deaths on the rise among lung cancer patients especially in U.S.A. The development of non tyrosine kinase Inhibitors (Non-TKIs) anticancer agents with optimized reaction conditions, purification, solubility that can be eventually be used for the treatment of resistant NSCLC has gained prominent interest due to its ability to inhibit acquired resistance. With our successful synthesis of pyrazolone molecular hybrids via microwave assisted processes, 23-compound library series were screened against A549 cells and non-cancerous HLF cell lines using MTT assay. Significantly, our screening results on these cell lines especially cancerous A549 cells yielded 5 highly active and potent compounds which were screened on non-cancerous HLF cells at timed intervals of 24hrs, 48hrs, 72hrs to assess its toxicity and selectivity concentrations towards non-cancerous cells. #SML – 107 compound showed relatively good selectivity

towards non-cancerous HLF cells making it less toxic to the normal lung cells

40