Auburn Research Day 2022

Mason C McIntosh; Casey L. Sexton; Joshua S. Godwin; Bradley A. Ruple; Shelby C. Osburn; Blake R. Hollingsworth; Philip J. Agostinelli; Andreas N. Kavazis; C. Brooks Mobley; Tim N. Ziegenfuss; Hector L. Lopez; Varun B. Dwaraka; Ryan Smith; Kaelin C. Young, PhD; Michael D. Roberts Auburn University; The Center for Applied Health Sciences, Canfield, OH; TruDiagnostic, Lexington, KY; Edward Via College of Osteopathic Medicine-Auburn Campus B i omed i ca l Resea rch | Med i ca l St udent Effects Of Different Types Of Resistance Exercise Failure Training On The Methylation Status Of Genes That Drive Skeletal Muscle Hypertrophy 5

Objective: We sought to determine how one bout of resistance training to failure with either higher repetitions (30FAIL) or lower repetitions (80FAIL) affected the promoter methylation statuses of genes that drive skeletal muscle hypertrophy. Hypotheses: We hypothesized that a bout of 80FAIL training would lead to a more robust hypomethylation of genes that regulate skeletal muscle hypertrophy compared to 30FAIL training. Methods: Eleven previously-trained college-aged men (age: 23 ± 4 years, 11.42 ± 6.38 percent fat, 4 ± 3 years training experience) volunteered for this study. Each participant underwent two training bouts (spaced one week apart) involving either: i) 30FAIL training; 4 sets of back squats and 4 sets of leg extensors to failure at 30% of one-repetition maximum (1RM), or: ii) 80FAIL training; 4 sets of both exercises at 80% of 1RM. Muscle biopsies from the vastus lateralis were collected prior to each bout (PRE), 3 hours following each bout (3hPOST), and 6 hours following each bout (6hPOST). Following the conclusion of the study, tissue was batch-processed for DNA isolation, and DNA was subjected to the Illumina MethylationEPIC array. In an a priori fashion, genes that have been shown to induce skeletal muscle hypertrophy in genetic mouse models were the target of this investigation (Verbrugge et al. Frontiers Physiol, 2019).

Results: Total training volume (sets x reps x load) between the 30FAIL and 80FAIL bouts were not significantly different (p= 0.571). Differentially methylated region changes for the following genes from PRE to 3hPOST and PRE to 6hPOST are presented herein: SKI, FST, AKT1, ACVR2B, MSTN, KLF10, RHEB, IGF1, PAPPA, PPARD, IKBKB, FSTL3, ATGR1, UCN3, MCU, JUNB, NCOR1, GPRASP1, GRB10, MMP9, DGKZ, PPARGC1A, SMAD4, LTBP4, BMPR1A, CRTC2, XIAP, DGAT1, THRA, ADRB2, ASB15, CAST, EIF2B5, BDKRB2, TPT1, NR3C1, NR4A1, GNAS, PLD1, CRYM, CAMKK1, YAP1, INHBA, TP53INP2, INHBB, NOL3, and ESR1. Additionally, significant differences between conditions at each time point are highlighted. Conclusions: This study continues to display how different modalities of resistance training affect the skeletal muscle molecular milieu and furthers our scientific understanding of factors that contribute to training adaptations.

Cl i n i ca l Case Repor t | Med i ca l St udent Abnormal Presentation of Multiple Myeloma in Pregnancy

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Robert Chory OMS III; Kiveum Kim OMS III; Ryan Cone OMS III; Joshua Hollingsworth PhD, PharmD; Rowell Ashford, MD VCOM-Auburn Campus, Princeton Baptist Medical Center

Multiple myeloma is a malignancy with an estimated prevalence in the United States of 1 in 132 (0.76%). The condition is three times more common in African-American populations when compared to Caucasians, and is classically a disease of the elderly, with only 10% of cases being diagnosed before age 50. The only known risk factors for MM are high BMI, family history of MM and exposure to agent orange in patients with Monoclonal Gammopathy of Unknown Significance. Classic presentation of multiple myeloma in the general population includes lytic bone lesions, elevated calcium levels, renal injury, and a normochromic, normocytic anemia which was the most common finding. Here we report a rare case of multiple myeloma in a 43-year-old African American woman at 24 weeks gestation, who presented for severe flank pain and difficulty breathing. Lytic bone lesions, anemia, and elevated total protein were noted and an iliac crest

biopsy confirmed the diagnosis of MM. She was started on steroids at 27 weeks gestation and delivered via caesarian-section at 30 weeks gestation. Postpartum, a treatment regimen to allow for breast-feeding was discussed but unable to be accommodated, and she was started on a multi drug chemotherapy regimen. There have only been about 30 cases of multiple myeloma in pregnant women reported in the literature. The rarity and lack of information on the effects of multiple myeloma in pregnancy was the primary indication for publication of this case.

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